pET-29b(+)_NSP4ss_SerM_NucA-His
(Plasmid
#218190)
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PurposeBacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 218190 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-29b(+)
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)T7 Express Iq lysY
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Growth instructionsFor expression, grow in your preferred BL21(DE3) or related strain. Induce with 0.5 mM IPTG at 18C overnight.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namenucA
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SpeciesSerratia marcescens
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Mutationcodon optimized ecoli residues - 22-266
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GenBank IDAAA26560.1 P13717
- Promoter T7
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Tags
/ Fusion Proteins
- NSP4 signal secretion peptide (N terminal on insert)
- His (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7promoter
- 3′ sequencing primer T7terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid expresses a nuclease. Leaky expression of the nuclease may degrade extracted plasmid DNA over time. It is recommended that this plasmid be maintained in the provided host strain. If plasmid is extracted, use it immediately.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET-29b(+)_NSP4ss_SerM_NucA-His was a gift from Florence Pojer (Addgene plasmid # 218190 ; http://n2t.net/addgene:218190 ; RRID:Addgene_218190)
Map uploaded by the depositor.
